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lines changed Original file line number Diff line number Diff line change @@ -38,6 +38,25 @@ is run in parallel using `scattergather` and is implemented in the
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needs to know ahead of time how many families to expect, which needs to be
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specified in the configuration file.
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+ ---
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+ ### ` makeblastdb `
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+
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+ Creates a BLAST database from all family FASTA files from the ` family_fasta `
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+ rule, retaining the sequences that have an OpenToL ID and reformatting them
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+ so that the definition line only has the process ID of the sequence. The
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+ script is implemented in [ makeblastdb.sh] ( scripts/makeblastdb.sh ) , which
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+ uses the ` makeblastdb ` command from the BLAST+ suite to create the database.
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+
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+ ---
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+ ### ` get_outgroups `
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+
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+ Fetches outgroup sequences from the BLAST database. This is done by running
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+ a BLAST search with the focal ingroup sequences (i.e. a family fasta file)
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+ against the BLAST database, and then selecting the most common hits across the
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+ ingroup sequences. The script is implemented in
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+ [ get_outgroups.sh] ( scripts/get_outgroups.sh ) and is parallelized using
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+ ` scattergather ` .
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+
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---
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### ` family_constraint `
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