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Experimental Design

Overview

Phenotyping

The TERRA-REF project is collecting phenotype data from 852 ****sorghum genotypes grown in three locations:

Data source MAC KSU Danforth
Scanalyzer Field System X
Scanalyzer 3D System X
Phenotractor X X
UAV (drone) X X
Manual field data X X

Genotyping

Whole genome resequencing is being carried out on ~400 sorghum accessions to understand the landscape of genetic variation in the selected germplasm and enable high-resolution mapping of bioenergy traits with genome wide association studies (GWAS). Additionally, ~200 sorghum recombinant inbred lines (RILs) will be characterized with ~400,000 genetic markers using genotyping-by-sequencing (Morris et al., 2013) for trait dissection in the RIL population and testcross hybrids of the RIL population.