diff --git a/packages/vite/src/node/__tests__/config.spec.ts b/packages/vite/src/node/__tests__/config.spec.ts
index e2966f262add71..82f4a3973085f8 100644
--- a/packages/vite/src/node/__tests__/config.spec.ts
+++ b/packages/vite/src/node/__tests__/config.spec.ts
@@ -513,3 +513,97 @@ test('config compat 3', async () => {
     ]
   `)
 })
+
+test('preTransformRequests', async () => {
+  async function testConfig(inlineConfig: InlineConfig) {
+    return Object.fromEntries(
+      Object.entries(
+        (await resolveConfig(inlineConfig, 'serve')).environments,
+      ).map(([name, e]) => [name, e.dev.preTransformRequests]),
+    )
+  }
+
+  expect(
+    await testConfig({
+      environments: {
+        custom: {},
+        customTrue: {
+          dev: {
+            preTransformRequests: true,
+          },
+        },
+        customFalse: {
+          dev: {
+            preTransformRequests: false,
+          },
+        },
+      },
+    }),
+  ).toMatchInlineSnapshot(`
+    {
+      "client": true,
+      "custom": false,
+      "customFalse": false,
+      "customTrue": true,
+      "ssr": false,
+    }
+  `)
+
+  expect(
+    await testConfig({
+      server: {
+        preTransformRequests: true,
+      },
+      environments: {
+        custom: {},
+        customTrue: {
+          dev: {
+            preTransformRequests: true,
+          },
+        },
+        customFalse: {
+          dev: {
+            preTransformRequests: false,
+          },
+        },
+      },
+    }),
+  ).toMatchInlineSnapshot(`
+    {
+      "client": true,
+      "custom": true,
+      "customFalse": false,
+      "customTrue": true,
+      "ssr": true,
+    }
+  `)
+
+  expect(
+    await testConfig({
+      server: {
+        preTransformRequests: false,
+      },
+      environments: {
+        custom: {},
+        customTrue: {
+          dev: {
+            preTransformRequests: true,
+          },
+        },
+        customFalse: {
+          dev: {
+            preTransformRequests: false,
+          },
+        },
+      },
+    }),
+  ).toMatchInlineSnapshot(`
+    {
+      "client": false,
+      "custom": false,
+      "customFalse": false,
+      "customTrue": true,
+      "ssr": false,
+    }
+  `)
+})
diff --git a/packages/vite/src/node/config.ts b/packages/vite/src/node/config.ts
index 9c2e36cd9d9ea3..0d9dcffce4973b 100644
--- a/packages/vite/src/node/config.ts
+++ b/packages/vite/src/node/config.ts
@@ -739,12 +739,13 @@ export function resolveDevEnvironmentOptions(
   consumer: 'client' | 'server' | undefined,
   // Backward compatibility
   skipSsrTransform?: boolean,
+  preTransformRequest?: boolean,
 ): ResolvedDevEnvironmentOptions {
   const resolved = mergeWithDefaults(
     {
       ...configDefaults.dev,
       sourcemapIgnoreList: isInNodeModules,
-      preTransformRequests: consumer === 'client',
+      preTransformRequests: preTransformRequest ?? consumer === 'client',
       createEnvironment:
         environmentName === 'client'
           ? defaultCreateClientDevEnvironment
@@ -775,6 +776,7 @@ function resolveEnvironmentOptions(
   // Backward compatibility
   skipSsrTransform?: boolean,
   isSsrTargetWebworkerSet?: boolean,
+  preTransformRequests?: boolean,
 ): ResolvedEnvironmentOptions {
   const isClientEnvironment = environmentName === 'client'
   const consumer =
@@ -806,6 +808,7 @@ function resolveEnvironmentOptions(
       environmentName,
       consumer,
       skipSsrTransform,
+      preTransformRequests,
     ),
     build: resolveBuildEnvironmentOptions(
       options.build ?? {},
@@ -1196,6 +1199,7 @@ export async function resolveConfig(
       environmentName,
       config.experimental?.skipSsrTransform,
       config.ssr?.target === 'webworker',
+      config.server?.preTransformRequests,
     )
   }