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Fix 08207f #4871

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56 changes: 30 additions & 26 deletions protocols/08207f/08207f.ot2.apiv2.py
Original file line number Diff line number Diff line change
@@ -81,17 +81,19 @@ def slow_withdraw(pip, well, delay_seconds=2.0):
dest_well = dest_plate.wells_by_name()[well]
volume = float(row[7])

if volume > 20:
if not p300.has_tip:
p300.pick_up_tip()
p300.aspirate(volume, buffer.bottom(h_buff))
p300.dispense(volume, dest_well)

else:
if not p20.has_tip:
p20.pick_up_tip()
p20.aspirate(volume, buffer.bottom(h_buff))
p20.dispense(volume, dest_well)
if volume > 0:

if volume > 20:
if not p300.has_tip:
p300.pick_up_tip()
p300.aspirate(volume, buffer.bottom(h_buff))
p300.dispense(volume, dest_well)

else:
if not p20.has_tip:
p20.pick_up_tip()
p20.aspirate(volume, buffer.bottom(h_buff))
p20.dispense(volume, dest_well)

adjust_height(volume)

@@ -110,18 +112,20 @@ def slow_withdraw(pip, well, delay_seconds=2.0):
volume = float(row[6])
mix_reps = 2

if volume > 20:
p300.pick_up_tip()
p300.aspirate(volume, source_well)
p300.dispense(volume, dest_well)
p300.mix(mix_reps, 20)
p300.blowout()
p300.drop_tip()

else:
p20.pick_up_tip()
p20.aspirate(volume, source_well)
p20.dispense(volume, dest_well)
p20.mix(mix_reps, 10)
p20.blowout()
p20.drop_tip()
if volume > 0:

if volume > 20:
p300.pick_up_tip()
p300.aspirate(volume, source_well)
p300.dispense(volume, dest_well)
p300.mix(mix_reps, 20)
p300.blowout()
p300.drop_tip()

else:
p20.pick_up_tip()
p20.aspirate(volume, source_well)
p20.dispense(volume, dest_well)
p20.mix(mix_reps, 10)
p20.blowout()
p20.drop_tip()
2 changes: 1 addition & 1 deletion protocols/08207f/fields.json
Original file line number Diff line number Diff line change
@@ -22,7 +22,7 @@
},
{
"type": "dropDown",
"label": "Labware on the Temperature Module",
"label": "Labware for Samples on the Temperature Module",
"name": "labware_temp_deck",
"options": [
{ "label": "eppendorftwin.tec96_96_aluminumblock_150ul", "value": "eppendorftwin.tec96_96_aluminumblock_150ul" },
1,128 changes: 1,128 additions & 0 deletions protocols/08207f/labware/alphalaboratories_96_aluminumblock_200ul.json

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