negative extents to matrix

[parkjooyoung99]

Dear developer,

I am using FastMNN for batch correction. Normally, I used 2000 HVGs but now I am trying to use all genes in my matrix, and retrieve batch corrected expression matrix which is possible with scanpy.

However, I am getting the following error:
Error in rbind(...) : negative extents to matrix
Calls: RunFastMNN ... -> standardGeneric -> eval -> eval -> eval -> rbind

My code is :

msk = readRDS('mydata.rds')
msk=DietSeurat(msk)
idx = rownames(msk@assays$RNA@data)[which(rowSums(msk@assays$RNA@data) != 0)]
print(idx)
count = msk@assays$RNA@counts[idx,]
meta = [email protected]
rm(msk)
msk = CreateSeuratObject(count, meta.data = meta)
msk = NormalizeData(msk)
msk = RunFastMNN(object.list = SplitObject(msk, split.by = "subtype"), features = rownames(msk))

If you need test data, I will send the link to you.
Thank you!